Biochemical characterization of rice glutelin

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August undefined, 2024

WebDec 1, 2024 · A spectrofluorometric method was utilized to provide additional information about the interaction of rice glutelin and gallic acid. Fluorescence excitation was carried out at 280 nm, since this wavelength is sensitive to changes in the local environments of tryptophan and tyrosine groups, which provides indirect information about alterations in … WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable …

Solubility and conformational characterization of rice …

WebJul 5, 2024 · Background Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the … WebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported … how fast is mach 5 in meters per second

Rice Glutelins SpringerLink

WebBiochemical Characterization of Rice Glutelin1 Received for publication September 25, 1984 and in revised form January 14, 1985 TUAN-NAN WEN AND DAWN S. LUTHE* … WebApr 1, 2002 · Very little is known about the gene that regulates the biochemical and cellular processes responsible for the formation of PBs. Rice mutants that show variations in the profile patterns of storage proteins have been identified and assigned into four classes (Kumamaru et al., 1988; Ogawa et al., 1989). WebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation … high end sennheiser microphones for concert

Characterization of rice glutelin fibrils and their effect on

Characterization of binding interaction between rice glutelin …

WebSep 1, 2012 · The Osborne method comprising of a 2-step in vitro digestion of rice flour was used to extract albumin, globulin, prolamin, and glutelin by using pepsin and trypsin. Subsequently, the antioxidant … Expand WebThe rice (Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/α-2 encoded by glu4 while forming a new polypeptide of pI6.50/α-1. Experiments were conducted to identify the … high end senior living seattleWebLikeoatglobulin (1), rice glutelin appears to be preferentially synthesized on membrane-bound polysomes (15, 29) and it is packaged in protein bodies (29). In addition, recent data indicates that ... high end selling cars

"WebMay 1, 1985 · The two major subunits of rice glutelin, the acidic (α) and basic (β) polypeptides were purified by chromatofocusing and cation exchange … " - Biochemical characterization of rice glutelin

Biochemical characterization of rice glutelin

(PDF) Biochemical and molecular characterization of a rice …

WebJun 15, 2009 · In this study, transgenic a123 expressing glutelin with a mutation at the processing site proved to be useful for studying the glutelin sorting and assembly system in rice seeds. The results obtained using transgenic rice plants clearly address the functional role of post-translational processing from the glutelin precursor to the mature acidic ...

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WebNov 1, 2024 · Characterization of rice DDGS and WS2.2.1. Biochemical characterization. The particle size of DDGS was reduced with mortar and pestle, thereby passing it to 0.5 mm sieve to obtain uniform size of the sample. ... Glutelin and prolamin were the major protein fraction available. A significant percentage of protein was … WebJun 1, 2003 · L. Q. Qu 81-298-38-8373 81-298-38-8397 [email protected] X. L. Wei H. Satoh T. Kumamaru M. Ogawa F. Takaiwa Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan …

WebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin … WebEnter the email address you signed up with and we'll email you a reset link.

WebRice glutelin cDNA clones isolated from a rice cDNA library constructed from immature endosperm encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. SummaryRice glutelin cDNA clones were isolated from a rice cDNA library … WebJul 1, 2003 · An extensive analysis of the complete rice genome sequence indicates that there are at least 11 glutelin genes (Katsube-Tanaka et al., 2004). However, only GluA …

WebThe rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis …

WebJan 1, 1992 · The 51-kDa protein is the rice glutelin precursor, which is processed to give rise to the 34-37-kDa u-subunit and 21-23-kDa subunit [3,7]. Similarly, the 14-kDa protein has been identified as a rice prolamine [7]. The 25- and 16-kDa proteins were the most abundant polypeptides in the globulin fraction (Fig. 1, lane C). ... Biochemical ... how fast is mach 6 in ktsWebDec 31, 2024 · Enhancing the solubility of rice glutelin in neutral aqueous solution is the prerequisite for the development of rice protein drinks and ingredients. Herein, glutelin … how fast is mach 60 in mphWebEurope PMC is an archive of life sciences journal literature. how fast is mach 6.70 in mphWebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … how fast is mach 5 mphWebSep 1, 2024 · Rice glutelin has high asparagine and glutamine contents (Liu et al., 2011). Thus, it likely has high ability to form fibril aggregates, since Tang et al. (2010) … highendservice.netWebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C. how fast is mach .80WebUnlike other cereals which accumulate prolamins as their primary nitrogen reserve, the major class of storage proteins in rice and oat are the glutelins and 12S globulins, respectively. These proteins are highly homologous at the primary sequence level to the leguminous US globulins. This similarity in protein structure extends to the molecular ... high end senior living communities