Ctll 2 proliferation assay

Author: tugu

August undefined, 2024

Webinterleukin-15, antagonist, peptide, serum, CTLL-2 cell line, proliferation assay. ABSTRACT Dysregulated expression of Interleukin (IL)-15 has been associated to several autoimmune and inflammatory diseases. In this sense, some agents that inhibit IL-15 activity have been developed as possible drugs to treat these pathologies. WebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human …

CTLL-2 93042610 Sigma-Aldrich

WebTitle: CTLL-2 Proliferation Assay for Determination of Bioactivity of Recombinant Human Interleukin 2 (rhIL-2) UNCONTROLLED COPY FOR TRAINING AND REFERENCE … WebAsn77 of IL-15 undergoes deamidation with a t 1/2 of ~4 days at pH 7.4, 37°C, but the IL-15 (Asp77) product is equipotent in the CTLL-2 bioassay and <5% additional degradation occurred over 8 days. 27 28 Here, when IL-15 was kept at pH 7.4, 37°C, for 10 days, there was no significant change in EC 50 in cell-based IL-2Rβ/γc dimerization or ELISA … biosmart portable heater reviews

重组人白介素2Recom_价格-品牌-详情介绍_丁香通

WebDownload scientific diagram CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 ... WebAug 31, 2009 · A colorimetric cell proliferation assay using soluble tetrazolium salt [(CellTiter 96(R) Aqueous One Solution) cell proliferation reagent, containing the (3-(4,5 … WebApr 11, 2024 · Recombinant Mouse IL2 (Catalog # 402 ML) stimulates cell proliferation of the CTLL 2 mouse cytotoxic T cell line. The ED 50 for this effect is 0.1 0.4 ng/mL. 6'6 3$*(1 μg/lane of Recombinant Mouse IL2 was resolved with SDSPAGE under reducing (R) conditions and visualized by silver staining, biosmart testing centre

A universal reporter cell line for bioactivity evaluation of …

WebApr 14, 2024 · Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ KÉ 3URWHLQ6'6 3$*( 2 μg/lane of Recombinant Human ILKÉ2 Protein (Catalog # BT-002) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and … biosmart on webWebOct 31, 2000 · In this study, alamarBlue was used to measure the proliferation of the IL-2-dependent cytotoxic T cell line, CTLL-2. The colorimetric change of alamarBlue at 570 nm compared to the reference wavelength, 600 nm, was proportional to the number of viable cells. The sensitivity of the IL-2 assay using ... proliferation assays. PDF; Standard … bio smartschool

"WebProliferation: IL-2 : CTLL-2 (ATCC TIB-214) 2: 24: 10: ≤175pg/mL: Proliferation: IL-3 : TF-1 (ATCC CRL-2003) 2: 48: 10: ≤0.6-0.15ng/mL: Proliferation: IL-4 : TF-1 (ATCC CRL-2003) 2: 48: 10: ... Cytotoxicity Assay: VEGF 121: HUVEC: 2: 72: 1000: ≤8-10ng/mL: Proliferation: Mouse Cytokine Bioassay Quick Guide Cytokine Indicator Cells Cell ... " - Ctll 2 proliferation assay

Ctll 2 proliferation assay

FUSION PROTEINS TARGETING TUMOUR ASSOCIATED …

Webfrom the Mo7e and CTLL-2 cyto-kine-dependent proliferation assay, PFC-1 exhibited an approx-imately 10-fold stronger potency than rhIL-15 in PBMC prolifera-tion, while 10 nM rhIL-15 led to a proliferation rate of 20.71% and 1 nM PFC-1 resulted in a mean value of 22.05% (Fig. 2B). The enhanced activity could be due to the higher activity of IL ... WebApr 5, 2024 · measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL. Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-FreeTM Recombinant Human IL-2. %LRDFWLYLW\

Did you know?

WebThe bioactivity of the superagonist was characterized by CTLL-2 cells proliferation assay in vitro, showing its capability of stimulating the expansion of memory CD8 + T cells (cluster of differentiation) in mouse spleen. WebThe biological activity of the recovered soluble rhIL-2 protein was determined in a proliferation assay using CTLL-2 cells. Human IL-2 purchased from Sigma-Aldrich Co. …

WebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. 培地 RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL … WebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents.

WebFor example, Recombinant Human IL-2 can activate proliferation of IL-2 dependent cell lines, CTLL-2 and HT-2. The same lot of recombinant protein provides different ED 50 values depending on the cell line used for stimulation. In the CTLL-2 stimulation assay, the ED 50 is 0.26 ng/ml corresponding to a specific activity of 3.73 x10 6 units/mg. WebFeb 16, 2024 · CCK-8 assay TGF-β could inhibit the IL-2 dependent proliferation of CTLL-2 and HT-2. We performed CCK-8 assays to measure the capability of YM101 antagonizing the effect of TGF-β. CTLL-2 and HT-2 cells (1 × 10 3 per well) were seeded in 96-well plates (3599, Corning). Then, TGF-β1 and antibodies (10 5 pM) were added into plates.

Web2.2. CTLL-2 cell proliferation assay Brieﬂy, CTLL-2 cells were cultured in RPMI 1640 sup-plemented with 10%fetal bovineserum (FBS),and200U/ml IL-2. The cells were harvested in their logarithmic phase (Cell passage 5 …

WebJun 2, 2012 · A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the ability to … dairy queen swartz creekWebMay 24, 2015 · Cell Proliferation Assay Application of a colorimetric CTLL-2 cell proliferation assay for the evaluation of IL-15 antagonists Authors: Yunier Rodríguez Center for Genetic Engineering and... biosmart heater with air purifierWebApr 13, 2024 · of Recombinant Human IL-2 as measured in a cell proliferation assay CTLL 2 mouse cytotoxic T cells (orange, green, red, respectively). 6'6 3$*(5HFRPELQDQW+XPDQ,/ 3URWHLQ6'6 3$*( 1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver … biosmart heater reviewsWebTitle: CTLL-2 Cell Proliferation Assay for Determination of Bioactivity of IL-15 . This procedure is made available through federal funds from the National Cancer Institute, … biosmart testinghttp://www.wrenshallab.com/uploads/5/6/3/8/56385051/mttctll2.pdf biosmb-pd-ld100bWebCTLL-2 is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon Interleukin-2 (IL-2) for growth and can be used to assay for IL-2. … bios megatrends usb bootWebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa. dairy queen sugar free ice cream