Electroporation of phaeodactylum tricornutum

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WebA highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi … WebJan 1, 2024 · Here we describe a high-efficiency nuclear transformation method for the model diatom Phaeodactylum tricornutum using an electroporation system, and the maximum transformation frequency...

Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum ...

WebApr 1, 2024 · In this study, the correlation between the specific growth rate of Phaeodactylum tricornutum and fucoxanthin (fucoxanthin) biosynthesis was investigated both in batch and in semi-continuous cultures. Fucoxanthin content from P. tricornutum biomass showed a positive correlation with specific growth rate during the … untitled wyatt earp anthology

Electroporation Transformation Protocol for …

WebDec 4, 2012 · As concerns the classical microalgal transformation systems referred, Chlamydomonas and Phaeodactylum, there is a wide collection of strong constitutive homologous promoters, such as the rubisco small subunit RBCS2 promoter fused to the heat shock protein (HSP70A) promoter of C. reinhardtii or the fucoxanthin chlorophyll … WebApr 7, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to... WebAug 20, 2024 · Phaeodactylum tricornutum is a model diatom with defined culture conditions, but routine genetic manipulations are hindered by a lack of simple and robust genetic tools. ... In contrast to ... untitled wrestling game

Highly efficient transformation of the diatom Phaeodactylum tricornutum ...

Phaeodactylum tricornutum - an overview ScienceDirect Topics

WebAug 30, 2024 · An efficient nuclear transformation method has been established for the pennate marine diatom Phaeodactylum tricornutum using an electroporation system … WebAug 27, 2024 · The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), … recliner sofa mechanism partsWebCurrently, training through the Biochemistry PhD program in Dr. Karas's lab, which focuses on developing innovative strategies for genome engineering. I study Phaeodactylum tricornutum, a eukaryotic micro-algae, and have created a platform for rapid organelle genome engineering in this model system. My research interests include: 1) organelle … recliner sofa living room arrangement

"WebHolly L. Prentice's 9 research works with 530 citations and 118 reads, including: Mutations in the Schizosaccharomyces pombe heat shock factor that differentially affect responses to heat and ... " - Electroporation of phaeodactylum tricornutum

Electroporation of phaeodactylum tricornutum

Phaeodactylum tricornutum - an overview ScienceDirect Topics

WebJan 11, 2024 · Episome-Based Gene Expression Modulation Platform in the Model Diatom Phaeodactylum tricornutum (Journal Article) DOE PAGES DOE PAGES ® Journal Article: Episome-Based Gene Expression Modulation Platform in the Model Diatom Phaeodactylum tricornutum Episome-Based Gene Expression Modulation Platform in … WebApr 23, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells.

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WebPhaeodactylum tricornutum (CCAP1055/1) Perkinsus marinus (ATCC PRA240) Oxyrrhis marina (CCMP 1788, CCMP 1795) Alexandrium catenella (CCMPBF-5) Karlodinium veneficum (CCMP1975) Amphidinium carterae (CCMP1314) Diplonema papillatum (ATCC 50162) Eutreptiella gymnastica (SCCAP K-0333) WebJun 1, 2012 · The promoter/terminator of the nitrate reductase gene of the model diatom Phaeodactylum tricornutum was cloned and used to drive chloramphenicol …

WebHerein reported are new tricyclic cytidine compounds, such as 8-diethylamino-tC (8-DEA-tC), that respond to DNA and/or RNA duplex formation with up to a 20-fold increase in fluore WebSuccessful transformation of Phaeodactylum tricornutum (CMP632) by electroporation was achieved based on a modified version of Zhang & Hu (2014).

WebPhaeodactylum tricornutum (UTCC646). We attempted a trophic conversion of this alga by transforming it with genes encoding glucose transporters. The transporter genes used in-cluded Glut1 from human erythrocytes (12); Hup1 from the microalga Chlorella kessleri (13); and Hxt1, Hxt2, and Hxt4 from Saccha-romyces cerevisiae (14). The coding regions WebAug 1, 2014 · Miyahara et al. (2013) also reported electroporation transformation of P. tricornutum, however, the protocol applied a multi-pulse electroporation for the …

WebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium recipe (Guillard, 1975), rather standard in cultivation of marine diatoms, ingredients are shown in table 1. Figure 1. Phaeodactylum tricornutum Bohlin (1897). CCAP 1055/15.

WebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium … untitled x platesWebMay 26, 2009 · Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms’ main features that allowed using them ... untitled x mas filmWeb• Developed an electroporation protocol for gene transformation in diatoms. ... Quantification of chrysolaminarin from the model diatom Phaeodactylum tricornutum Algal research December 31, ... recliner sofa low heightWebJan 1, 2024 · In this study, the marine diatom Phaeodactylum tricornutum was selected as the model organism since it presents many advantages, including culture easiness and … recliner sofa motorised factoryWebApr 3, 2024 · The promoter/terminator of the nitrate reductase gene of the model diatom Phaeodactylum tricornutum was cloned and used to drive chloramphenicol acetyltransferase (CAT) reporter gene expression. The construct was transferred by … Pdf/Epub - Transformation of diatom Phaeodactylum tricornutum by … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that … recliner sofa navy fabricWebCell Size-specific Transfection by Micropillar Array Electroporation Xuan Liu, Yingbo Zu, and 385535540195. ELEKTROPORATIONSPROTOKOLLE: MIKROORGANISMUS, SÄUGETIERSYSTEM und Nanogerät von Sh - EUR 297,94. ZU VERKAUFEN! Cell Size-specific Transfection by Micropillar Array Electroporation Xuan Liu, Yingbo Zu, and … untitled yahooメールWebP. tricornutum strain CCAP1055/1 was used in this study. The LCYB sequence was amplified from cDNA synthesized from total RNA, and cloned into plasmid pPha-T1 [9]. P. tricornutum transformation was carried out using a simple electroporation protocol described by Zhang and colleagues [10]. For the analysis of growth conditions effects on ... recliner sofa motorised manufacturers