Hif 1a antibody
WebFigure 1: Western Blot - Anti-HIF1A Antibody (A11945) Western blot analysis of extracts of various cell lines, using Anti-HIF1A Antibody (A11945) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes).
Hif 1a antibody
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WebKnockout Validated Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, Dual ISH-IHC + more. Reactivity: … WebI've had good luck with HIF-1a antibody Cat#3716 from Cell Signaling Tech. As previously suggested hypoxic chamber helps, however, if you do it quickly you don't need the chamber.
WebHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation … http://www.abways.com/showproduct.asp?cid=CY5197
WebHIF-1 alpha Antibody detects endogenous levels of total HIF-1 alpha. Immunogen: A synthesized peptide derived from human HIF-1 alpha. Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses ... WebBiological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. Simple Western: HIF-1 alpha Antibody [NB100-449] - …
Web保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保 …
WebLane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less ... (HIF-1). HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. flower mart baltimore 2022WebTo determine whether HIF-1α binds these HREs, we performed a CHIP assay using anti-HIF-1α antibodies in A549 cells cultured under hypoxic (1.5% O 2) or normoxic (21% O 2) conditions. DNA fragments containing the HRE1 and/or HRE2 binding sites were significantly more abundant under hypoxic conditions ( Figure 4G ), suggesting that HIF … flower mound 10 day forecastWeb保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 flower kissing ballWeb23 de jul. de 2024 · Datasheets. HIF1a Antibody (28b) is a mouse monoclonal IgG 1 κ HIF1a antibody, cited in 241 publications, provided at 200 µg/ml. epitope mapping within … flower pastelWebSee all HIF-1 alpha primary antibodies Description Mouse monoclonal [H1alpha67] to HIF-1 alpha Host species Mouse Tested applications Suitable for: IP, Flow Cyt, ICC/IF, WB … flower mound country clubWeb5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional … flower of evil ep 15 eng subWebHIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an … flower pick machine