WebMar 26, 2015 · The shortest wavelength variants of this series, iRFP670 and iRFP682 showed maximal excitation with visible red lasers. The longer wavelength variants iRFP702, iRFP713 and iRFP720 could be optimally excited by NIR lasers ranging from 685 to 730 nm. ... allowed spectra of all five iRFPs to be analyzed simultaneously with no spectral overlap ... WebThe spectral and biochemical properties of the iRFP670, iRFP682, iRFP713 proteins and …
Near-Infrared Fluorescent Proteins Engineered from Bacterial ...
WebApr 27, 2024 · 118 very narrow emission spectra with maxima at 670 nm and 520 nm for iRFP670 and Alexa Fluor 119 488, respectively (Fig. 1C). 120 To produce viral particles for the rectal challenge experiments, 293T cells were co-121 transfected with the LI670 vector and a SIV3 packaging construct. This provided the viral WebTOPO iRFP670 (Plasmid #68443) Print Enlarge View all sequences Purpose TOPO construct expressing iRFP670 for generation of GMAP-compatible gene Depositing Lab Tyler Jacks Publication Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836. ( How to cite ) Sequence Information Sequences (3) Ordering births per 1000 women
Molecular Basis of Spectral Diversity in Near-Infrared …
WebWe developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. WebIn miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights... WebJan 1, 2024 · We chose mTagBFP2 (blue), mNeonGreen (green), mApple (red), and iRFP670 (near Infrared) for which the spectra match with the classic excitation laser lines (405 nm, 488 nm, 561 nm, 640 nm) and CHROMA® Quad bandpass filter 89100bs, so that we can conduct fast 4-color imaging without switching between filters and minimizing crosstalk … births per 1000 population