Orc2 antibody drosophila
WebApr 13, 2024 · This study shows that Drosophila germ granules, which initially protect RNAs, subsequently become sites of selective mRNA degradation; this plasticity supports germ cell development. ... 50 μg/mL tRNA), embryos were incubated with primary antibody in PBHT overnight at 4°C with rocking. Primary antibodies used: 1:250 rabbit anti-CCR4 , 1:250 ... WebAug 30, 2011 · ORC function is required to allow both stages of replication initiation at DAFC-34B. DNA copy number was examined in a female-sterile allele of ORC, orc2fs293, which reduces gene amplification. orc2k43 is a null allele. Amplification in the sibling heterozygotes shows typical amplification levels.
Orc2 antibody drosophila
Did you know?
WebOct 9, 2024 · Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterized by microtia, primordial dwarfism, small ears and skeletal abnormalities. … WebIn Drosophila, ORC localizes to regions of open chromatin with contributions from activating histone modifications, DNA sequence, DNA binding proteins, and nucleosome remodelers …
WebJun 24, 2004 · The centrosomes of HeLa cells arrested in the aberrant mitotic state failed to label with anti-Orc2 antibodies, suggesting that the Orc2 centrosome labeling was specific (Supplementary Figure S10B). ... Mutations in Drosophila Orc2 also disrupt HP1 association with heterochromatin (Huang et al, 1998). WebTo verify the phosphorylation at Thr-116 and Thr-226 of ORC2, we generated phosphoantibodies that specifically detected phospho-Thr-116 or phospho-Thr-226 of ORC2. The α-pT116 and α-pT226 antibodies recognized GST-ORC2 expressed in 293T cells (Fig. 1C). However, neither phosphoantibody detected mutant forms of the protein with Thr to …
WebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we … WebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E).
WebAbout 20% of the Orc2 subunit is eluted from the nuclei after an RNase treatment ... anti-rabbit IgG (Amersham) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes) were used as secondary antibodies. Drosophila cell culture extracts. Drosophila Schneider line 2 (S2) cells were maintained at 25°C in Schneider's Insect Medium ...
WebJun 23, 2024 · The process is likely conserved since the human ORC2 WHD interacts with ORC1 in the ORC2-5 structure and occupies a number of dynamic positions 58. The Cdc6 WHD undergoes a series of movements ... in-company investigationWebNov 2, 2011 · We thank I. Chesnokov and the Bloomington Drosophila Stock Center (Bloomington, IN) for flies, S. Bell and M. Botchan for α-Orc2 antibodies, J. Powers of the Indiana Light Microscopy Imaging Center (Bloomington, IN) for helpful advice, and M. Lilly and members of the Calvi lab for advice and comments on the manuscript. We thank M. … incekum beach resort to airportWebWe isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is … in-communityWebGene: orc-2 Immunogen: Recombinant His6-tagged ORC-2 fusion protein Alternate Gene Names: Alternate Antibody Name: 4D4 Clonality: Monoclonal Alternate Antigen Name: … incel forocochesWebDec 1, 2024 · Protein A-Sepharose (#6501-5; BioVision) and rabbit polyclonal antibodies against Drosophila Orc2 subunit were added and incubated with the supernatant overnight. Sepharose beads were washed three times with low-salt immunoprecipitation buffer and diluted in 10 µl of immunoprecipitation buffer. incekum strandWebNational Center for Biotechnology Information incekum beach resort recenzeWebPreparation of the ORC2 antibodies. The Drosophila ORC2 coding sequence was PCR amplified from a 4- to 8-hr embryonic cDNA library (Brown and Kafatos 1988). The PCR product was subcloned into pCYB2 (New England Biolabs) usingNdeI and SmaI sites engineered into the termini of the PCR product. DmORC2 protein was expressed in … incek loft sitesi