Phis2.1 vector
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Phis2.1 vector
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http://www.biofeng.com/zaiti/jiaomu/pYES2.html WebbStandard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) ... The 1-base overhangs …
Webb8 juli 2024 · pHis2 编号 载体名称 北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息 出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体 高拷贝/低拷贝: -- 启动子: -- … WebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription …
WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates … WebbpHIS2 vector (V001297#) Basic Vector Information. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The …
Webb24 jan. 2024 · For ligation: the molar ratio of your bait sequence: vector ranges from 3:1 and 5:1. The reaction can be put at 16 degrees overnight (in PCR cycler machine). It …
Webb质粒简介 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with … stephen king and peter straub the talismanWebb1 年: 应用: 酵母双 ... pHIS2 vector酵母单杂交克隆载体 2μg MF2354-1000UL Y187 Chemically Competent Cell 酵母菌化学感受态细胞 10×100μl MF2356-1000UL AH109 Chemically Competent Cell酵母菌化学感受态细胞 10×100μl MF2358-1000UL EGY48 Chemically Competent ... stephen king address in bangor maineWebb20 feb. 2024 · The full-length cDNA of TcbZIP60 was cloned into the pGADT7 vector used for homologous recombination, and the promoter sequences of TcCHS and TcAOC were cloned separately into the pHis2.1 vector. The resultant pGADT7: TcbZIP60 plasmid was co-transformed into yeast strain Y187, together with pHis2.1: TcCHS or pHis2.1: TcAOC , … pioneer woman boarding house phone numberhttp://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf stephen king a face in the crowd pdfWebb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … stephen kimble casehttp://www.yrgene.com/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf stephen king accidentWebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … stephen king and wife